James M. Bradeen Potato Pathology and Genomics
Our Potato Pathology & Genomics Program focuses on the genetics and genomics of potato plant resistances and important potato disease organisms. Using molecular and applied research techniques, we are identifying, characterizing, isolating, and deploying previously unknown or under-utilized sources of natural genetic resistance. Utilizing wild potato germplasm, we aim for long-term solutions to important potato diseases, while reducing grower and environmental costs associated with frequent pesticide applications. Current research includes the development of novel genomics tools for potato and related wild species, including genome-wide mapping methods for potato disease resistance genes and "allelic mining" at important disease resistance loci, screening wild potato species for resistance to late blight and Verticillium wilt, and evaluation of in vitro screening procedures for late blight.
Recent Publications: Mazourek, M., E. Cirulli, S.M. Collier, L.G. Landry, B.-C. Kang, E.A.
Quirin, J.M. Bradeen, P. Moffett, and M. Jahn. 2009. The fractionated
orthology of Bs2 and Rx/Gpa2 and the comparative
genomics model of disease resistance in the Solanaceae. Genetics
182:1351-1364.
Bradeen, J.M., M. Iorizzo,
D.S. Mollov, J.
Raasch, L. Colton Kramer, B.P. Millett, S. Austin-Phillips, J. Jiang,
and D.
Carputo. 2009. Higher copy numbers of the potato RB
transgene correspond to enhanced transcript and late blight
resistance levels. Molecular
Plant-Microbe Interactions 22: 437-446.
Millett, B.P., D.S. Mollov,
M. Iorizzo, D.
Carputo, and J.M. Bradeen. 2009. Changes in disease resistance
phenotypes
associated with plant physiological age are not caused by variation in
R gene
transcript abundance. Molecular
Plant-Microbe Interactions 22: 362-368.
Bradeen, J.M., D. Carputo,
and D.S. Douches
2008. Potato In: Kole, C. and Hall,
T.C. (eds.) Transgenic Sugar, Tuber and Fiber Crops Vol. 7. (Oxford,
UK:
Wiley-Blackwell). 117-156.
Bradeen,
J.M. and D.S. Mollov. 2007. Herbicide tolerance in primitive diploid
potato
species comprising superseries Stellata:
towards establishment of seedling cultivation conditions for field
evaluations.
American Journal of Potato Research.
84: 415-424.
Aversano,
R., M.R. Ercolano, L. Frusciante, L. Monti, J.M. Bradeen, G.
Cristinzio, A.
Zoina, N. Greco, S. Vitale, and D. Carputo. 2007. Resistance traits and
AFLP
characterization of diploid primitive tuber-bearing potatoes. Genetic Resources and Crop Evolution.
54: 1797-1806.
Millett,
B.P., and J.M. Bradeen. 2007. Development of allele-specific PCR and
RT–PCR
assays for clustered resistance genes using a potato late blight
resistance
transgene as a model. Theoretical and
Applied Genetics 114: 501-513.
Bradeen,
J.M., and P.W. Simon. 2007. Carrot. In: C. Kole (ed.) Genome Mapping
and
Molecular Breeding in Plants. Berlin: Springer-Verlag. pp. 161-184.
Whitaker,
V.M., J.M. Bradeen, and S.C. Hokanson. 2007. Distribution of rose black
spot (Diplocarpon rosae) genetic diversity in
eastern North America using amplified fragment length polymorphism and
implications for resistance breeding. Journal
of the American Society for Horticultural Science 132:534-540.
Whitaker,
V.M., K. Zuzek, S.C. Hokanson, and J.M. Bradeen. 2007. Culturing and
long-term
storage of virulent races of the rose blackspot pathogen, Diplocarpon
rosae Wolf. Acta
Horticulturae 751:199-205.
Zlesak, D.C.,
J.M. Bradeen, and N.O. Anderson. 2007. The use of
AFLP markers to resolve clonal origin and integrity in rose, hydrangea,
and
lily. Floriculture and Ornamental
Biotechnology 1:51-60.
Sanchez, M.
J. and J. M. Bradeen. 2006. Towards
efficient isolation of R gene
orthologs from multiple genotypes: optimization of Long Range-PCR. Molecular Breeding. 17:137-148.
Song, J.*, J. M. Bradeen*,
S. K. Naess, J. A.
Raasch, S. M. Wielgus, G. T. Haberlach, J. Liu, H. Kuang, S.
Austin-Phillips,
C. R. Buell, J. P. Helgeson, and J. Jiang. 2003. Gene RB cloned
from Solanum
bulbocastanum confers broad spectrum resistance to potato late
blight. Proceedings
of the National Academy of Sciences. 100:9128-9133. *J. Song and J. M.
Bradeen contributed equally to this work and are considered “co-first
authors”. Bradeen, J. M., S. K.
Naess, J. Song, G. T.
Haberlach, S. M. Wielgus, C. R. Buell, J. Jiang, and J. P. Helgeson. 2003. Concomitant reiterative BAC
walking
and fine genetic mapping enable physical map development for the
broad-spectrum
late blight resistance region, RB. Molecular
Genetics and Genomics. 269:603-611. Song, J., J. M. Bradeen, S.
K. Naess, J. P.
Helgeson, and J. Jiang. 2003. BIBAC and TAC
clones containing potato genomic DNA fragments larger than 100 kb are
not
stable in Agrobacterium. Theoretical
and Applied Genetics. 107:958-964.
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