HOST-PARASITE GENETICS MODULE

 

PLANT PATHOLOGY 5102/8102

 

FALL SEMESTER 2004

 

 

Instructor:      Brian J. Steffenson

                        Office:  310 Stakman Hall

                        Phone:  (612) 625-4735

                        E-mail:  bsteffen@umn.edu

 

Office hours:  10:45-11:45 AM Monday and Wednesday and by appointment

 

Lectures:  9:30-10:45 AM on Monday and Wednesday in 365 Borlaug Hall

Discussion: 1:00-2:15 on Wednesday in 491 Borlaug Hall

 

Course web site: http://plpa.coafes.umn.edu/~bsteff/teaching5102.html

 

Credits: Three (3.0)

 

Course objectives:  My goal is to provide information on the basic genetic fundamentals of host-parasite interactions.  In this course, we will examine Mendelian, populational, and molecular genetic aspects of host-parasite interactions.  Pertinent examples will be given on the genetics of individual hosts and parasites.  Additionally, practical information will be presented to aid students in studying host-parasite systems and developing disease control strategies using genetic resistance.

 

Prerequisites:  Students must have a thorough understanding of the basic concepts of plant disease, mycology, genetics, and epidemiology.

 

Text:  None required.  Reference materials and assigned papers are required reading and are placed on electronic reserve or in the Plant Pathology library.  To access the electronic reserve, go to http://reserve.lib.umn.edu/cgi-bin/eres/; then click on “View Courses and Documents;” type in your x500 number/password and click on “Submit”; next click either “Department Name,” “Instructor’s Last Name,” or “Search” to navigate to the course reserve list of “PlPa5102 STEFFENSON Epidemiology and Host-Parasite Genetics.”  Click on this text and you will then be asked to provide a password.  The password is marave7.  Click “Accept” at the bottom to open the electronic reserve list for the course.  The articles are saved as PDF files and are organized in alphabetical order by the senior author’s last name.  In some cases, a single article is divided into several smaller PDF files to hasten download time.  You may elect to save a tree by not printing the article and reading it on the computer.

 

Course requirements:  One mid-term exam is tentatively scheduled for November 17.  The final examination is scheduled for December 20 from 10:30-12:30 AM. 

 

In addition to these exams, students will be required to write an abbreviated grant proposal on a topic dealing with any aspect of host-parasite genetics as per the general guidelines of Section 51.8: Biology of Plant-Microbe Associations from the USDA National Research Initiative Competitive Grants Program (USDA-NRICGP).  This proposal is to be written using the exact format and guidelines of the USDA-NRICGP. 

 

For essential information, go to the following websites:

The funding opportunities section of CSREES-NRI Competitive Grants Program is at:  http://www.csrees.usda.gov/funding/nri/nri.html 

 

For a complete description of the grant proposal sections, click on “NRI Request for Proposals.”  Here you can download text or PDF versions of the proposal guidelines.  Within this document, see pages 55-57 for information about Section 51.8: Biology of Plant-Microbe Associations. Pages 72—79 contain a detailed description on how to write each section of the grant. 

 

Make absolutely sure that you follow the exact directions for each section of the grant proposal.  For example, in the Rationale and Significance section, you must demonstrate how your objectives will help contribute to the potential long-range improvement in and sustainability of U.S. (or world agriculture) or to one or more of the research objectives described under Applicable Regulations in the NRI (see Part I, B. Purpose, Priorities and Fund Availability on pages 7-8).

 

For proposal application forms, click on http://www.csrees.usda.gov/funding/forms.html

From this website, you will download and complete the following: 1) the proposal cover page (page 3 of forms document), 2) the proposal abstract (page 5), and 3) the proposal budget (page 6). 

 

Your complete grant proposal document will include in this order:

1) Proposal Cover Page (1 page: Form CSREES-2002 from forms website)

2) Table of Contents (1 page)

3) Project Summary (1 page: Form CSREES-2003 from forms website)

4) Project Description (must not exceed 10 pages)

5) References to Project Description (must not exceed 5 pages)

6) Project Budget (1 page: Form CSREES-2003 from forms website)

6) Appendices (optional)

The entire grant proposal must not exceed 20 pages.

 

The grant proposal exercise will be evaluated based on the criteria outlined on pages 90-91 of the proposal guidelines.

 

Each student will present his or her grant proposal (oral presentation of 10-11 minutes maximum) to the class on December 15.  Some will give their presentations in the morning lecture period, the others in the afternoon discussion section. 

 

Students should not submit grant proposals on topics related to current or previous research projects or current or previous grant proposals required in other classes.  If such a topic is used for this grant proposal, the maximum grade attainable is 70%. 

 

Class participation is encouraged.  The “homework” for 5102 enrollees will include the grant proposal.  In contrast, the “homework” for 8102 enrollees will include the grant proposal and the discussion section (see below).

 

Fifty percent of your final course grade is based on material covered during the last half of the semester. 

 

The breakdown in grading for the Host-Parasite Genetics module is as follows:

 

Grading:        

Mid-term exam:                                                         25%  November 17

Final exam:                                                                 30%  December 20

Grant Proposal                                                           30%  Due: December 17 at 5:00PM

Participation in Discussion                                        10%  Every Wednesday

Grant Proposal Presentations                                     5%  December 15

 

The University of Minnesota Uniform Grading and Transcript Policy can be found at http://www1.umn.edu/usenate/policies/gradingpolicy.html.

 

Grant Proposal:  General topics for possible grant proposals are given below.  The instructor must approve the final topic for your proposal.  Students are required to submit a brief outline of their proposal on November 3.  The grant proposals are due on December 17 at 5:00 PM.  Late papers will be penalized 25% of their final value per day.  No exceptions!

 

1)  Genetics and molecular mapping of disease resistance in the host

2)  Stabilizing selection

3)  Expression profiling of host-parasite interactions

4)  Gene deployment strategies for managing pathogen populations

5)  Fitness attributes in pathogens

6)  Genetic systems in plant pathogenic fungi, bacteria, nematodes, or viruses

7)  Fungicide resistance in fungi or antibiotic resistance in bacteria

8)  Genetics and molecular mapping of pathogenicity/virulence in the host

9)  Disease as a factor in plant evolution

10) Genetic vulnerability in crops

11) Host-parasite coevolution

12) Population genetics of host-parasite interactions

13) Wild species as sources of disease resistance and alien gene transfer

14) Hypovirulence and hypoparasitism

15) Risk assessment of transgenic plants in the environment

16) Risk assessment of transgenic microbes in the environment

17) Molecular basis and mechanisms of host resistance

18) Mechanisms of variability in pathogenic fungi, bacteria, nematodes, and viruses.

19) Durable resistance

20) Molecular determinants of pathogenicity/virulence

21) Vector specificity in the transmission of viruses

22) Molecular signaling in host-parasite interactions

 

 

Academic misconduct:  The Conduct Code of the University of Minnesota details matters of scholastic dishonesty to include the following: submission of false records of academic achievement; cheating on assignments or examinations; plagiarizing; altering, forging or misusing a University academic record; taking, acquiring or using test materials without faculty permission; acting alone or in cooperation with another to falsify records or to obtain dishonestly grades, honors, awards, or professional endorsement.  Academic dishonesty in any form will not be tolerated.

Disability access:  The University of Minnesota policy is to provide reasonable accommodations to students who have disabilities that may affect their ability to participate in course activities or to meet course requirements.  Students with disabilities should contact me within the first week of the course to discuss specific needs.  An additional resource is the Disability Services Office (Phone: 624-4037; http://ds.umn.edu/)

 

Discrimination and sexual harassment: The University of Minnesota is committed to the policy that all persons shall have equal access to its programs, facilities, and employment without regard to race, religion, color, sex, national origin, handicap, age, or veteran status.  Questions regarding these issues should be directed to the appropriate officer in the university's equal opportunity office (419 Morrill Hall, Phone: 624-9547) or see http://www.eoaffact.umn.edu/.